eSupervisor Detail

About me

Expertise in non-coding RNA biology and cancer chemoresistance, with a strong interest in expanding my knowledge in molecular biology, cell biology, and drug discovery. Experienced in elucidating mechanisms of chemoresistance and miRNA/piRNA-mediated regulatory networks in oral squamous cell carcinoma. Passionate about contributing to both fundamental and translational research aimed at overcoming drug resistance and advancing cancer therapeutics using molecular biology techniques.

Interests: Molecular and RNA Biology Techniques — miRNA isolation, quantification, and functional analysis Gene expression profiling (qRT-PCR, RNA expression analysis) miRNA mimic/inhibitor transfection and functional validation Molecular cloning, protein purification and site-directed mutagenesis Lentiviral-mediated gene knockdown and overexpression systems. Protein and Cellular Mechanism Studies — "Western blotting and protein expression analysis Co-immunoprecipitation (protein protein interactions) Immunofluorescence-based localization studies Cellular trafficking and mitochondrial function assays Cell Culture and Cancer Biology Techniques — Mammalian cell culture (2D and 3D spheroid tumor models) Chemoresistance model development (cisplatin-resistant OSCC cells) Drug response assays (MTT, viability assays) Flow cytometry for cellular functioning and viability analysis Microscopy and Imaging — Confocal microscopy Fluorescence microscopy Immunofluorescence imaging for subcellular localization Drug Discovery and Translational Techniques — Molecular docking and in silico drug screening Drug repurposing approaches targeting miRNA-regulated pathways Protein-drug interaction analysis Functional validation of candidate therapeutics Computational and Analytical Skills — Integration of computational and experimental pipelines for target identification Data analysis using GraphPad Prism and Microsoft Excel Statistical analysis (hypothesis testing, regression, significance testing) Interpretation of miRNA and gene expression data Specialized Research Expertise — miRNA-mediated regulation of cancer chemoresistance Non-coding RNA biology (miRNA and piRNA) in cancer OSCC (Oral Squamous Cell Carcinoma) drug resistance mechanisms. RNA-guided therapeutic targeting and drug discovery Personal & Professional Skills — Strong scientific writing skills Experience in manuscript preparation, revision, and peer-review responses Ability to design hypothesis-driven experiments independently Effective collaboration in interdisciplinary research teams Strong presentation skills Time management and multitasking across parallel research projects