eSupervisor Detail

About me

I am a PhD graduate in Molecular Biology with expertise in molecular microbiology, bacterial genetics, and DNA repair. My research involved transcriptional regulation, gene cloning, mutant construction, qPCR, EMSA, protein purification, and data analysis. I can offer students research guidance in experiment designing and data analysis, scientific writing support for proposal, research paper or thesis writing, and mentorship to build strong experimental and critical thinking skills.

Interests: My research interests lie in understanding the molecular mechanisms that govern bacterial stress responses, DNA repair pathways, and antimicrobial resistance. During my doctoral research, I focused on deciphering the regulatory networks involved in DNA damage repair in cyanobacteria, with particular emphasis on the coordinated roles of transcriptional regulators and recombination proteins. This work provided me with extensive experience in molecular microbiology, bacterial genetics, and functional genomics, while strengthening my interest in exploring how microorganisms adapt to environmental and cellular stress. My PhD research investigated the regulation of homologous recombination genes involved in DNA repair and genome stability. Using a combination of molecular biology, microbiological, and biochemical approaches, I characterized regulatory interactions controlling the expression of key DNA repair genes. My work involved gene cloning, construction of deletion and overexpression mutants, promoter activity analysis, protein purification, electrophoretic mobility shift assays (EMSA), quantitative real-time PCR, RNA isolation, and stress tolerance studies. These experiments enabled me to understand how transcriptional regulators coordinate bacterial responses to DNA damage and environmental stress, contributing to the maintenance of genomic integrity. Building upon this foundation, I am keen to expand my research towards clinically relevant bacterial pathogens and investigate the molecular basis of antimicrobial resistance. I am particularly interested in understanding how mobile genetic elements, such as plasmids, facilitate the acquisition, maintenance, and dissemination of antibiotic resistance genes within bacterial populations. Exploring plasmid biology, bacterial adaptation, and host-plasmid interactions offers exciting opportunities to identify novel strategies for limiting the spread of multidrug-resistant bacteria. My long-term research goal is to integrate molecular genetics, microbiology, and genomic technologies to address fundamental questions in bacterial physiology and pathogenesis. I am interested in applying advanced approaches, including transcriptomics, comparative genomics, genome editing, and high-throughput sequencing, to unravel complex regulatory networks governing bacterial survival under stress conditions. I also aspire to investigate bacterial evolution, plasmid dynamics, and host-pathogen interactions to better understand mechanisms that contribute to persistence and antimicrobial resistance. Beyond fundamental research, I am motivated by the translational potential of microbiology. I hope to contribute to the development of innovative therapeutic strategies, antimicrobial stewardship, and improved management of infectious diseases by identifying molecular targets that can be exploited to combat antibiotic-resistant pathogens. Working in an interdisciplinary and collaborative research environment will allow me to broaden my technical expertise while contributing meaningfully to projects addressing global public health challenges. Overall, my research experience has provided a strong foundation in molecular microbiology, bacterial genetics, and microbial physiology. I am eager to undertake postdoctoral research that combines mechanistic studies with modern genomic and biochemical approaches to advance our understanding of bacterial biology and antimicrobial resistance. Through continued research, I aim to contribute to scientific discoveries that improve our understanding of microbial adaptation while supporting the development of effective strategies to address the growing threat of antimicrobial resistance.