eSupervisor Detail

Specialized consulting for researchers working with embryonic stem cells (ESCs), pluripotent stem cells, and differentiation protocols. Drawing from 8 years of hands-on experience in stem cell fate specification and lineage commitment, this program provides expert guidance on experimental design, protocol optimization, and troubleshooting complex differentiation challenges. Areas of Expertise: ESC Culture & Maintenance: Feeder-free culture systems, quality control, pluripotency marker validation. Directed Differentiation: Neural lineage differentiation protocols, mesoderm specification, germ layer commitment strategies. Lineage Analysis: Flow cytometry-based characterization, immunocytochemistry for differentiation markers, transcriptional profiling. Genetic Manipulation: CRISPR-mediated gene editing in stem cells, shRNA knockdown approaches, gain/loss of function studies Mechanistic Studies: Cell fate specification signaling pathways (Wnt, BMP, FGF, Nodal), epigenetic regulation, translational control mechanisms. Research Design: Experimental planning for developmental biology questions, deubiquitinating enzyme (DUB) functional studies

Comprehensive troubleshooting service for molecular biology experiments that aren't working as expected. With expertise across cloning, gene expression analysis, protein studies, and functional assays, this program helps researchers identify bottlenecks, optimize protocols, and achieve reproducible results. Particularly valuable for labs facing persistent technical challenges or establishing new assay workflows. Technical Expertise: Molecular Cloning - Molecular cloning strategies and vector design, Golden gate, GIBSON assembly, Primer design for qRT-PCR and sequencing, RNA extraction and cDNA preparation troubleshooting, qRT-PCR optimization and data normalization, shRNA-mediated knockdown and CRISPR/Cas9 gene editing. Protein Analysis - Western blot optimization (antibody selection, blocking conditions, signal enhancement), Immunoprecipitation protocol development, Protein expression and purification in bacterial systems, Protein stability assays and biochemical characterization. Immunoassays & Cell-Based Techniques - Flow cytometry panel design and gating strategies, Immunocytochemistry and immunohistochemistry optimization, Confocal microscopy sample preparation and imaging parameters, ELISA development and validation, Cell viability and proliferation assay troubleshooting. Advanced Techniques – CHIP, Cut & Run assay for chromatin studies, Nanoparticle Tracking Analysis (NTA), Multi-parameter quantitative analysis and statistical validation. Consultation Services: Step-by-step protocol review and gap analysis. Root cause analysis for failed experiments, Reagent and equipment recommendations, Positive/negative control design, Statistical analysis planning and data interpretation.