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Project Scientist

Dr. Avinash Kumar

Biological Network Analysis

Uttar Pradesh, India
2 Years
Joined Dec 2025
Indian Institute Of Technology Kanpur (Iit Kanpur)
Department Of Biological Sciences And Bioengineering At Iit Kanpur
0 Consultations

About me

Project Scientist with a Ph.D. in Biotechnology and strong expertise in protein biochemistry, enzymology, and protein purification. Experienced in high-resolution mass spectrometry, electrophoresis, chromatography, and advanced bioanalytical techniques. Proven ability to design, execute, and manage independent research projects, mentor students, and contribute to high-quality scientific publications.

Interests: Protein biochemistry and enzymology, Protein purification and characterization, Seed storage proteins and legume proteomics, Amyloid aggregation and protein self-assembly, Structure–function relationships of proteins, High-resolution mass spectrometry–based proteomics, Nanobiotechnology and biofunctional materials, Enzyme stability and protein engineering, Bioanalytical and biophysical techniques

My Services

Protein Biophysical Characterization Techniques - Research Decode Consultancy Cover Image

Protein Biophysical Characterization Techniques

Protein Biophysical Characterization Techniques1. Spectroscopic TechniquesCircular Dichroism (CD) spectroscopyUV–Visible spectroscopyIntrinsic fluorescence spectroscopy (Trp/Tyr)Extrinsic fluorescence spectroscopy (ANS, ThT, Nile Red)Fluorescence lifetime measurementsFourier Transform Infrared (FTIR) spectroscopyRaman spectroscopyNear-UV CD (tertiary structure)2. Hydrodynamic & Size-Based TechniquesDynamic Light Scattering (DLS)Static Light Scattering (SLS)Analytical Ultracentrifugation (AUC)Size Exclusion Chromatography (SEC)SEC–MALS (Multi-Angle Light Scattering)3. Structural & Conformational AnalysisX-ray crystallographyCryo-Electron Microscopy (Cryo-EM)Nuclear Magnetic Resonance (NMR) spectroscopySmall-Angle X-ray Scattering (SAXS)Small-Angle Neutron Scattering (SANS)4. Amyloid & Aggregation-Specific TechniquesThioflavin T (ThT) fluorescence assayCongo Red binding and birefringenceTransmission Electron Microscopy (TEM)Atomic Force Microscopy (AFM)Turbidity (light scattering) assaysSeeding and aggregation kinetics analysis5. Thermodynamic & Stability MeasurementsDifferential Scanning Calorimetry (DSC)Differential Scanning Fluorimetry (DSF / Thermofluor)Thermal denaturation (CD/fluorescence-based)Chemical denaturation (urea, GdnHCl)6. Surface & Interaction StudiesIsothermal Titration Calorimetry (ITC)Surface Plasmon Resonance (SPR)Bio-layer Interferometry (BLI)Microscale Thermophoresis (MST)7. Redox & Chemical State AnalysisFree thiol estimation (Ellman’s assay)Disulfide bond analysisRedox titration assaysOxidation–reduction switching studies8. Electrophoretic & Complementary MethodsSDS-PAGENative PAGEBlue Native PAGEAgarose gel electrophoresis (aggregates)

₹140

Session

₹800

Project
Dr. Avinash Kumar

Dr. Avinash Kumar

2 Years experience