eSupervisor Detail

Bioinformatics:Molecular Docking: Structure-based drug design (SBDD), high-throughput virtual screening of ligand libraries (HTVS), and protein-protein/protein-DNA interaction mapping.Molecular Dynamics (MD) Simulations: Stability analysis, binding free energy calculations (MM-GBSA/PBSA), and conformational landscape exploration over microsecond timescales.Advanced Analytics: Lead optimization, and ADMET prediction to bridge the gap between computational models and wet-lab validation.Full manuscript drafting (Introduction + Methods + Results + Discussion) : ₹ Extra 8000

Bioinformatics Data analysis:Comprehensive Differential Gene Expression (DEG) analysis services for high-throughput sequencing datasets, including:RNA-seq (mRNA sequencing)miRNA-seqCRISPR sgRNA screen dataWith 6+ years of experience in transcriptomic data analysis, I offer end-to-end analytical support — from raw FASTQ files to biological interpretation and publication-ready results.Analysis✔ Quality control (FastQC, MultiQC)✔ Adapter trimming & filtering (fastp/Trimmomatic)✔ Alignment (HISAT2/STAR) or pseudoalignment (Salmon)✔ Read quantification (featureCounts/HTSeq)✔ Normalization & DEG analysis (DESeq2/edgeR/limma)✔ PCA & clustering✔ Volcano plots & heatmaps✔ Functional enrichment (GO/KEGG)✔ Pathway analysis✔ Publication-ready figuresCRISPR Screen Analysis✔ sgRNA count normalization✔ Identification of enriched/depleted genes (MAGeCK)✔ Gene ranking and hit identification✔ Pathway enrichment analysis of candidate genes✔ Visualization of screening resultsDeliverablesDEG tables (Excel + CSV format)Statistical summary (log2FC, adjusted p-values)High-quality figures (PNG/PDF)Detailed ReportComprehensive analysis of Whole Exome Sequencing (WES) data for somatic mutation detection, commonly used in cancer genomics studies to identify tumor-specific variants by comparing tumor and matched normal samples.Analysis Workflow✔ Raw sequencing quality assessment (FastQC, MultiQC)✔ Adapter trimming and quality filtering (fastp / Trimmomatic)✔ Alignment to reference genome (BWA-MEM)✔ Sorting and indexing of BAM files✔ Duplicate marking (Picard MarkDuplicates)✔ Base Quality Score Recalibration (GATK BQSR)✔ Somatic variant calling (GATK Mutect2 / Strelka2 / VarScan2)✔ Variant filtering (FilterMutectCalls)Variant Annotation✔ Functional annotation (ANNOVAR / VEP)✔ Identification of somatic SNPs and INDELs✔ Variant classification (missense, nonsense, frameshift, splice-site)✔ Population frequency filtering (gnomAD, 1000 Genomes)✔ Cancer database annotation (COSMIC, ClinVar)Downstream Analysis✔ Identification of tumor-specific mutations✔ Mutation burden analysis✔ Visualization and reporting of somatic variantsDeliverables✔ Annotated somatic variant tables (VCF + Excel format)✔ Variant impact summaries✔ Mutation statistics and QC reports✔ Publication-quality figures✔ Detailed analysis reportPricing (depending on sample size and complexity)RNA-seq (Standard DEG):₹15,000 – ₹30,000miRNA-seq: ₹18,000 – ₹35,000CRISPR sgRNA Screen:₹18,000 – ₹35,000Whole Exome Somatic Variant Analysis: ₹25,000 – ₹45,000***EXTRA ₹8000 Full manuscript drafting (Introduction + Methods + Results + Discussion)Exome Sequencing Data Analysis (Somatic Variant Calling)